Human Bone Marrow Hosts Polyfunctional Memory CD4+ and CD8+ T Cells with Close Contact to IL-15-Producing Cells

Herndler-Brandstetter,D.; Landgraf,K.; Jenewein,B.; Tzankov,A.; Brunauer,R.; Brunner,S.; Parson,W.; Kloss,F.; Gassner,R.; Lepperdinger,G.; Grubeck-Loebenstein,B.
Recently, a key role in memory T cell homing and survival has been attributed to the bone marrow (BM) in mice. In the human BM, the repertoire, function, and survival niches of CD4(+) and CD8(+) T cells have not yet been elucidated. In this study, we demonstrate that CD4(+) and CD8(+) effector memory T cells accumulate in the human BM and are in a heightened activation state as revealed by CD69 expression. BM-resident memory T cells produce more IFN-gamma and are frequently polyfunctional. Immunofluorescence analysis revealed that CD4(+) and CD8(+) T cells are in the immediate vicinity of IL-15-producing BM cells, suggesting a close interaction between these two cell types and a regulatory role of IL-15 on T cells. Accordingly, IL-15 induced an identical pattern of CD69 expression in peripheral blood CD4(+) and CD8(+) T cell subsets. Moreover, the IL-15-inducible molecules Bcl-x(L), MIP-1alpha, MIP-1beta, and CCR5 were upregulated in the human BM. In summary, our results indicate that the human BM microenvironment, in particular IL-15-producing cells, is important for the maintenance of a polyfunctional memory CD4(+) and CD8(+) T cell pool
J.Immunol. 2011 186(12):6965-6971
Tags: ACTIVATION; Aging; analysis; Austria; blood; Bone Marrow; cell; EXPRESSION; human; Memory; Mice
PubMed: 21562158
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