Mechanistic aspects of nucleic-acid oxidation studied with electrochemistry-mass spectrometry

Oxidation reactions play a major role in the modification of nucleobases in DNA and RNA. Enzymatic oxidation reactions are involved in the control of epigenetic signaling as part of DNA-demethylation pathways. Oxidative stress gives rise to non-enzymatic oxidation. Many different oxidative DNA modifications have been identified. The cellular responses to such oxidative damage involve several processes, such as DNA repair, cell-cycle arrest and apoptosis. Persistent DNA damage may result in genomic instability, which is considered to play a role in the development of cardiovascular and neurological diseases, aging and cancer. Due to the involvement of nucleic-acid oxidation in many biological processes, understanding the underlying mechanisms is of the utmost importance. Herein, we demonstrate the vast potential of electrochemistry coupled to liquid chromatography-mass spectrometry as a tool for studying the oxidative stability of nucleic-acid species and identifying important oxidation products (C) 2014 Elsevier B.V. All rights reserved.
Trac-Trends in Analytical Chemistry 2015 70:100-111
Tags: DNA damage; DNA methylation; electrochemistry; liquid chromatography; mass spectrometry; oxidation; oxidative stress; radical cation; reactive oxygen species; rna damage; pyrolytic-graphite electrode; guanine radical cations; DNA-damage; cellular-DNA; deoxyribonucleic acid; redox potentials; purine-bases; guanosine; 5-hydroxymethylcytosine; 5-methylcytosine;
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