"Omics" Applications of Electrochemistry Coupled to Mass Spectrometry - A Review

Redox reactions are integral parts of many cellular processes. They are therefore extensively studied in vitro and in vivo. Electrochemistry (EC) represents a purely instrumental approach to characterize direct and indirect effects of redox reactions on bioorganic molecules, such as peptides, proteins, and endogenous and exogenous small molecules as well as nucleic acids. In addition to direct infusion electrospray ionization (ESI)-mass spectrometry (MS), hyphenated techniques such as liquid chromatography-mass spectrometry (LC-MS) are often applied for comprehensive characterization of reaction mixtures generated by EC experiments. EC-LC-MS represents a fast, automatable, and "green" approach that can be used in a variety of "omics" disciplines. This review highlights important trends and recent developments.
Lc Gc Europe 2015 28:138-150
Tags: oxidative drug-metabolism; electrospray ion-source; liquid chromatography/mass spectrometry; polycyclic aromatic-hydrocarbons; disulfide-containing proteins; biological redox reactions; wave potential pulses; in-vitro; flow cell; phase-i;
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