Application of micro-computed tomography to microstructure studies of the medicinal fungus Hericium coralloides

Pallua,J.D.;Kuhn,V.;Pallua,A.F.;Pfaller,K.;Pallua,A.K.;Recheis,W.;Poder,R.;
The potential of 3-D nondestructive imaging techniques such as micro-computed tomography (micro-CT) was evaluated to study morphological patterns of the potential medicinal fungus Hericium coralloides (Basidiomycota). Micro-CT results were correlated with histological information gained from scanning electron microscopy (SEM) and light microscopy (LM). It is demonstrated that the combination of these imaging methods results in a more distinct picture of the morphology of the edible and potentially medicinal Hericium coralloides basidiomata. In addition we have created 3-D reconstructions and visualizations based on micro-CT imagery from a randomly selected part of the upper region of a fresh H. coralloides basidioma: Analyses for the first time allowed an approximation of the evolutionary effectiveness of this bizarrely formed basidioma type in terms of the investment of tissue biomass and its reproductive output (production of basidiospores).
Mycologia 2015 107:227-238
Tags: evolutionary effectiveness; hericium coralloides; hymenophoral surface area; hyphal texture; micro-computed tomography; 3-d reconstructions; sholl analysis; spinal-cord; ct; iodine; model; soil; microtomography; reconstruction; optimization; segmentation;
PubMed: 25376797
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