STRSeq: A catalog of sequence diversity at human identification Short Tandem Repeat loci

Gettings,K.B.;Borsuk,L.A.;Ballard,D.;Bodner,M.;Budowle,B.;Devesse,L.;King,J.;Parson,W.;Phillips,C.;Vallone,P.M.;
The STR Sequencing Project (STRSeq) was initiated to facilitate the description of sequence-based alleles at the Short Tandem Repeat (STR) loci targeted in human identification assays. This international collaborative effort, which has been endorsed by the ISFG DNA Commission, provides a framework for communication among laboratories. The initial data used to populate the project are the aggregate alleles observed in targeted sequencing studies across four laboratories: National Institute of Standards and Technology (N=1786), Kings College London (N=1043), University of North Texas Health Sciences Center (N=839), and University of Santiago de Compostela (N=944), for a total of 4612 individuals. STRSeq data are maintained as GenBank records at the U.S. National Center for Biotechnology Information (NCBI), which participates in a daily data exchange with the DNA DataBank of Japan (DDBJ) and the European Nucleotide Archive (ENA). Each GenBank record contains the observed sequence of a STR region, annotation ("bracketing") of the repeat region and flanking region polymorphisms, information regarding the sequencing assay and data quality, and backward compatible length-based allele designation. STRSeq GenBank records are organized within a BioProject at NCBI (https://www.ncbi.nlm.nih.gov/bioproject/380127), which is sub-divided into: commonly used autosomal STRs, alternate autosomal STRs, Y-chromosomal STRs, and X-chromosomal STRs. Each of these categories is further divided into locus-specific BioProjects. The BioProject hierarchy facilitates access to the GenBank records by browsing, BLAST searching, or ftp download. Future plans include user interface tools at strseq.nist.gov, a pathway for submission of additional allele records by laboratories performing population sample sequencing and interaction with the STRidER web portal for quality control (http://strider.online).
Forensic Sci Int Genet 2017 31:111-117
Tags: Alleles; DNA/genetics; *Databases, Nucleic Acid; Forensic Genetics/standards; High-Throughput Nucleotide Sequencing; Humans; *Microsatellite Repeats; Polymorphism, Genetic; *Sequence Analysis, DNA; Terminology as Topic; *DNA sequencing; *Forensic STR; *Mps; *Ngs; *Nomenclature;
PubMed: 28888135
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